![]() View all charts in a single window for simplified data analysis and interpretationĪn example of analyzing HRM data using Bio-Rad's HRM software, Precision Melt Analysis™ software, is shown below.Analyze multiple experiments from a single plate.Share analysis settings among experiments.Display a plate view for easy identification of sample genotypes.Compare and combine data from multiple experiments by combining run results into a single melt study.HRM software is usually straightforward, and should allow users to: HRM analysis requires careful experimental design, HRM-specific reagents, and a sensitive real-time PCR instrument with software that can handle large quantities of data. In addition to the typical software packages, some instruments also offer HRM software to genotype samples based on their DNA thermal denaturation properties. Select Export to Excel in the data analysis window. Select Save Image As in the data analysis window.įig. You can also create reports or real-time PCR data markup language (RDML) files for quick import into qbase+ software.įig. If these values are out of range, you will need to troubleshoot your experiment.Įxport results for publication - Quickly export any charts or tables by right clicking in the window and selecting Save Image As (Figure 3) or Export to Excel (Figure 4). The efficiency should be within 90–110% and the R 2 should be >0.990. Check the efficiency and R 2 of the standard curve. Easily view up to six different charts or tables, such as the amplification plot, standard curve, gene expression chart, plate layout, or melt peak with the Custom Data View tab (Figure 2). Plate setup in the Plate Editor window.Īnalyze the data - a data file is automatically generated after a run with the gene expression module. This can be done before, during, or after a run.įig. Set up the plate - indicate position of unknown, no template controls (NTCs), and standard curve samples on plate (Figure 1). An example of how to analyze a gene expression experiment in CFX Maestro software is shown below. Similar to other real-time PCR software, CFX Maestro software offers several analysis modules, including quantification, melt curve, gene expression, allelic discrimination, and end-point analyses. With one software for all four systems, you can easily open and read files from any of the instruments, collaborate with other researchers, and move to another system without learning to use new software. Since you did not specify any other Assay IDs, the filenames are used.All of Bio-Rad's real-time PCR detection systems include the powerful, easy-to-use CFX Maestro Software. When you are finished importing, you'll see the runs grid showing the files you just uploaded. ![]() ![]() Click Save and Finish after the last file.The concentrations for standard wells will retain their values so you do not need to reenter them. Repeat the import process for the other two files, selecting the other two Run Data files in turn.On the next page, enter Concentrations for Standard Wells as shown here:.Make no other changes on this page, then click Next. In the Run Data field, click Choose File (or Browse) and select one of the sample data files you just downloaded.For Batch Properties, accept the default settings by clicking Next.In the ELISA Tutorial folder's Assay List, click the name you gave your assay design, here: ELISA Tutorial Assay.
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